Raw data of qRT-PCR and western blot analyses of proteasome subunits and GFP-CL1 degradation in Podospora anserina

2014-10-16T07:49:46Z (GMT) by Matthias Wiemer Heinz D. Osiewacz

Dataset 1 : Raw data of qRT-PCR analysis of the PaPre3 gene used in Figure 1A
CP values of the reference gene PaPorin and of the target gene PaPre3 are displayed for juvenile middle-aged and senescent samples

Dataset 2 : Raw data of qRT-PCR analysis of the PaPre2 gene used in Figure 1A
CP values of the reference gene PaPorin and of the target gene PaPre2 are displayed for juvenile middle-aged and senescent samples

Dataset 3 : Raw data of qRT-PCR analysis of the PaUmp1 gene used in Figure 1A
CP values of the reference gene PaPorin and of the target gene PaUmp1 are displayed for juvenile, middle-aged and senescent samples

Dataset 4 : Raw data of qRT-PCR analysis of the PaPre3 gene used in Figure 2A
CP values of the reference gene PaPorin and of the target gene PaPre3 are displayed. The wild type CP is the mean CP value of juvenile samples displayed in Dataset 1

Dataset 5 : Raw data of qRT-PCR analysis of the PaPre2 gene used in Figure 2B
CP values of the reference gene PaPorin and of the target gene PaPre2 are displayed. The wild type CP is the mean CP value of the juvenile samples displayed in Dataset 2

Dataset 6: Raw data of western blot displayed in Figure 1B probed with α-PaPRE2. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE2. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM (Cat# 26616) Prestained protein ladder. Lanes 2 – 7: Samples described in Figure 1B.

Dataset 7: Raw data of western blot displayed in Figure 1B probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 7 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 1 – 6: Samples described in Figure 1B.

Dataset 8: Raw data of western blot displayed in Figure 1B probed with α-PaPUP1. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPUP1. Red signal represents fluorescence at 700 nm. Lane 10 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 1 – 6: Samples described in Figure 1B. Lanes 7 - 9 are not relevant to this study.

Dataset 9: Raw data of western blot displayed in Figure 1B probed with α-PaHSP60. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-PaHSP60. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 2 - 7: Samples described in Figure 1B.

Dataset 10: Raw data of western blot displayed in Figure 2C probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 – 5 and lane 9: Samples described in Figure 1C. The other lanes are not relevant to this study.

Dataset 11: Raw data of western blot displayed in Figure 2D probed with α-PaPRE2. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE2. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 and 4: Samples described in Figure 1D. Lane 2 is not relevant to this study.

Dataset 12: Raw data of western blot displayed in Figure 2D probed with α-PaPRE3. Fluorescence was detected at 700 nm and 800 nm. Both signals are merged in the displayed image. Green signal represents fluorescence at 800 nm generated by anti-rabbit 800 antibody bound to α-PaPRE3. Red signal represents fluorescence at 700 nm. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 3 and 4: Samples described in Figure 1D. Lane 2 is not relevant to this study.

Dataset 13: Raw data of western blot displayed in Figure 3B probed with α-GFP. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-GFP. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 9 and 10: Samples described in Figure 3B.

Dataset 14: Raw data of western blot displayed in Figure 3C probed with α-GFP. Fluorescence was detected at 700 nm. Red signal represents fluorescence at 700 nm generated by anti-mouse 700 antibody bound to α-GFP. Lane 1 (from left to right): Thermo Fischer PageRulerTM Prestained protein ladder. Lanes 5 and 6: Samples described in Figure 3C.