Raw%20data%20experiments%20Lopez-Arias%20et%202017.xlsx (63.06 kB)
Raw data experiments Lopez-Arias et al 2017.xlsx
We sistematically compared the effect of neuronal expression of secretable forms of the amyloid peptides Abeta40, Abeta42 or Abeta42arc, with normal or elevated PI3K levels, on synaptic parameters of the Drosophila adult neuromuscular junction (NMJ), on brain neurodegeneration, and on longevity.
For SYNAPTIC PARAMETERS, the number of BRANCHES, BOUTONS, and SYNAPSES (Bruchpilot-positive active zones) was quantified in the adult abdominal ventral NMJ at different AGEs (days after eclosion).
WORKSHEET "SEGMENT AND AGE": data from wild type males of different ages (from 3 to 45 days after eclosion) in different SEGMENTS (A3, A4 and A5).
WORKSHEETS "NMJ+Abeta": data from A3 ventral NMJs of control males and males expressing each Abeta peptide in motor neurons, at 5 ages (3, 7, 15, 20 and 30 days after eclosion; at 30 days after eclosion, most Abeta42arc flies had dies, and therefore there is no data for this genotype/age). The box contains the key for the GENOTYPEs used. FLY# identifies each male with a unique number (with data from either the rigth or the left, or both, NMJs).
WORKSHEETS "NMJ+Abeta+PI3K": data from A3 ventral NMJs of control males and males co-expressing elevated PI3K levels and each Abeta peptide in motor neurons, at 2 ages (15 and 20 days after eclosion). The box contains the key for the GENOTYPEs used. FLY# identifies each male with a unique number (with data from either the rigth or the left, or both, NMJs).
WORKSHEET "NEURODEGENERATION": the percentage of neurodegenerated area (%NEURODEGENERATION) was measured at 15 and 20 days after eclosion (AGE) in whole brains from control males and males with pan-neural expression of each Abeta peptide and normal or elevated PI3K levels. The box contains the key for the GENOTYPEs used.
WORKSHEET "LONGEVITY OASIS": survival data from control males and males expressing each Abeta peptide with normal or elevated PI3K levels. Data are formatted for analysis with the online analysis survival package OASIS (doi:10.1371/journal.pone.0023525).The box contains the key for the GENOTYPEs used. For each genotype (%N), each line depicts the number of "dead" and "censored" males at a given age (#days).
For SYNAPTIC PARAMETERS, the number of BRANCHES, BOUTONS, and SYNAPSES (Bruchpilot-positive active zones) was quantified in the adult abdominal ventral NMJ at different AGEs (days after eclosion).
WORKSHEET "SEGMENT AND AGE": data from wild type males of different ages (from 3 to 45 days after eclosion) in different SEGMENTS (A3, A4 and A5).
WORKSHEETS "NMJ+Abeta": data from A3 ventral NMJs of control males and males expressing each Abeta peptide in motor neurons, at 5 ages (3, 7, 15, 20 and 30 days after eclosion; at 30 days after eclosion, most Abeta42arc flies had dies, and therefore there is no data for this genotype/age). The box contains the key for the GENOTYPEs used. FLY# identifies each male with a unique number (with data from either the rigth or the left, or both, NMJs).
WORKSHEETS "NMJ+Abeta+PI3K": data from A3 ventral NMJs of control males and males co-expressing elevated PI3K levels and each Abeta peptide in motor neurons, at 2 ages (15 and 20 days after eclosion). The box contains the key for the GENOTYPEs used. FLY# identifies each male with a unique number (with data from either the rigth or the left, or both, NMJs).
WORKSHEET "NEURODEGENERATION": the percentage of neurodegenerated area (%NEURODEGENERATION) was measured at 15 and 20 days after eclosion (AGE) in whole brains from control males and males with pan-neural expression of each Abeta peptide and normal or elevated PI3K levels. The box contains the key for the GENOTYPEs used.
WORKSHEET "LONGEVITY OASIS": survival data from control males and males expressing each Abeta peptide with normal or elevated PI3K levels. Data are formatted for analysis with the online analysis survival package OASIS (doi:10.1371/journal.pone.0023525).The box contains the key for the GENOTYPEs used. For each genotype (%N), each line depicts the number of "dead" and "censored" males at a given age (#days).
