RT–PCR analysis of cloned standards and mixed standards for

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Taken from "A real-time semi-quantitative RT–PCR assay demonstrates that the sequence dictates the frequency and characteristics of pilin antigenic variation in "

Nucleic Acids Research 2005;33(10):3363-3371.

Published online 9 Jun 2005

PMCID:PMC1148172.

© The Author 2005. Published by Oxford University Press. All rights reserved

() Representative fluorescence acquisition curves for hybridization probes with dilutions of standards (closed squares, 10 molecules; circles, 10 molecules; triangles, 10 molecules; and diamonds, 10). This is compared with pilin cDNA detected with hybridization probes (star). The opened star is the no RT control for contaminating DNA. The cross symbol is the no template control (background level). () Fluorescence acquisition curves for hybridization probes with dilutions of cloned standards (closed squares, 10 molecules; circles, 10 molecules; triangles, 10 molecules; and diamonds, 10) and dilutions of standards mixed with the 10 parental copy standard () (open squares, 10 molecules; circles, 10 molecules; triangles, 10 molecules; and diamonds, 10 molecules). Total pilin cDNA detected with hybridization probes is indicated by the star.