RPPA and Western blot confirmation of Smad protein phosphorylation.

<p>A) Phosphorylation status of nine RPPA targets after RVFV infection of HSAECs. Bar graph represents the mean fold change with standard deviation of three independent replicates. Phospho-specific Smad antibodies are noted as: pSmad1/5/9 MH2 [phosphorylated Smad1/5 (S463/465)/9 (S465/467)], pSmad1/5 MH2 [phosphorylated Smad1/5 (S463/465)], pSmad2 MH2 [phosphorylated Smad2 (S465/467)], and pSmad2 L [phosphorylated Smad2 (S245/250/255)]. Statistical significance of the phosphorylation changes are highlighted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191983#pone.0191983.t001" target="_blank">Table 1</a>. HSAEC (B) and Huh-7 cells (C) were infected at an MOI 5 with MP12 or ZH548, or mock infected (media alone). Protein lysates harvested at 3, 9, and 18hpi were analyzed for pSmad1/5/9 MH2, pSmad1/5 MH2, pSmad2 MH2, pSmad2 L, RVFV NP, Actin as well as total protein levels for Smad1, Smad5, and Smad2 by western blotting. Blots represent one replicate of three independent experiments. M:Mock; MP:MP12; ZH: ZH548.</p>