RNase L ANK repeats 1 and 2 are sufficient for interaction with L*.
2018-04-13T17:45:48Z (GMT) by
<p>A. Enhanced Green Fluorescent Protein (eGFP) was fused to the C-terminus of indicated Flag-tagged ankyrin repeats of mouse, rat and human RNase L. results of L* co-immunoprecipitation with eGFP constructs shown in B and C are summarized. NT: not tested. B. Co-immunoprecipitation of indicated Flag-tagged eGFP fusion proteins with HA-tagged L*DA. Right panels show Flag (ANK repeats) and HA (L*) detection after immunoprecipitation of HA. Left panels show detection of Flag and HA in cell lysates (Input). Similar results were obtained in 2 independent experiments. HC: immunoglobulin heavy chain, LC: immunoglobulin light chain. C. Co-immunoprecipitation of Flag-ANK R1-2-eGFP from indicated species with HA-tagged L* of DA or RTV-1. Upper panels show Flag and HA (L*) detection after immunoprecipitation of HA. Lower panels show detection of Flag and HA in cell lysates (Input). Shown are results representative of 2 independent experiments.</p>