RNA-Seq data of WT and Tssk6 KO spermatids
2017-04-05T13:17:46Z (GMT) by
Spermatids were purified from three sets of adult wild type (WT) and Tssk6 knockout (KO), total RNA was isolated, RNA-Seq libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kit, and sequenced on Illumina HiSeq 2500. Quality of raw data was assessed and passed by FastQC and reads were mapped to the reference genome (Mus musculus_UCSC_mm10). Cufflinks (v2.2.1) was used to estimate transcript levels represented by FPKM (fragments per kilo bases per million mapped reads) and differential expression between samples was determined by Cuffdiff (v.2.2.1). Student’s t-test was performed to calculate the P values.