RESTseq 1371054246_1946_00_800.jpg (38.79 kB)
RESTseq - principle of the sequencing approach
RESTseq - principle of the sequencing approach: genomic digestion, adapter ligation and then reduction of the library by digestion with secondary enzamyes and size selection --> the rest, the remaining uncleaved fragments of the corect size, can be directly sequenced. By combination of enzymes and size selection, all scales of numbers of fragments (and thus SNPs) can be reproduced.