Quantitative phase microscopy images of spherical droplets, beads, and cells

Example data sets for quantitative phase imaging.

These data were used by Müller et al. [1] to demonstrate the
accurate retrieval of size and refractive index for spherical
objects by means of a two-dimensional phase data fitting
algorithm.

Abbreviations:

- DHM: digital holographic microscopy
- FUS: fused in sarcoma (RNA-binding protein)
- HL60: name of a human leukemia cell line
- QLSI: quadri-wave lateral shearing interferometry
- PAA: Polyacrylamide (hydrogel)
- PBS: phosphate buffered saline (buffer medium)

The data are related to [1] according to the following scheme:

Figure 4:

a-c) SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07
Silicone oil droplet measured in PBS using QLSI
d-f) SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5
FUS protein droplet measured in egg extract using QLSI
(included in fig. 5a)
g-j) SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23
PAA hydrogel bead (referred to as "type A" in [1]) measured
in PBS using QLSI (included in fig. 5b)
k-m) DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11
PAA hydrogel bead (referred to as "type B" in [1]) measured
in PBS using DHM
n-q) QLSI_2015-11-09_16-03-52_HL60
HL60 cells measured in PBS using QLSI (included in fig. 5c)
r-s) DHM_2015-11-09_16-48-45_HL60
HL60 cells measured in PBS using DHM (included in fig. 5d)

Figure 5:

a) QLSI_2016-04-29_FUS-droplet-780-5
Time lapse measurement of a FUS protein droplet measured in
egg extract using QLSI
b) QLSI_2016-07-05_PAA-beads-S6-A
PAA hydrogel beads (referred to as "type A" in [1]) measured
in PBS using QLSI
c) QLSI_2015-11-09_HL60
HL60 cells measured in PBS using QLSI
d) DHM_2015-11-09_HL60
HL60 cells measured in PBS using DHM

The data are stored in different file formats:

- .tif: digital holography data or (!) SID4Bio (Phasics S.A.) data
- .h5: HDF5-based qpimage [2] file format
- .zip: each ZIP file contains several .tif files
- .cfg: meta data and analysis recipe for each data set,
stored as a DryMass [3] profile

The phase data can be extracted with DryMass [3] and qpformat [4],
e.g. using the command-line interface of DryMass:

dm_analyze_sphere --profile DHM_2015-11-09_HL60.cfg DHM_2015-11-09_HL60.zip

For more information and a detailed analysis, please refer to [1].


License: CC0 (https://creativecommons.org/publicdomain/zero/1.0/)


SHA256 sums:

cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_16-48-45_HL60.cfg
04eaf59da61934486385f42600aec0a2ffb3f71c93c2b6b6f247ac237088eefb DHM_2015-11-09_16-48-45_HL60.tif
cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_HL60.cfg
64e7b0e6fab4d947b82333ea2edd20b41ce51c813c97ae1070da90e58de35b02 DHM_2015-11-09_HL60.zip
685c886d44b9359dca1b2d2bd504f2b1d5f149c05da5a1263ee802c02fb41e66 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.cfg
75608e106ecf41a22d707c966faf850f1414e9f0012ce69104658680b4b26876 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.tif
138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_16-03-52_HL60.cfg
73dd8612b713b62227f52e68619f2e5794adabd29c09622b985fdcc842eb68ff QLSI_2015-11-09_16-03-52_HL60.h5
138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_HL60.cfg
9f3af6ccdd0a71e53d2907ee8f7b6e478c56da99d3dac9a443aceaa4b9f1658e QLSI_2015-11-09_HL60.h5
1a95d8bfcee3e4b442afccd88d0fa89ed9013fe0c96bf5a337ede481eef1f99d QLSI_2016-04-29_FUS-droplet-780-5.cfg
f9a407ca31f3aaa106aa7b5db74ab74b9244e9624212e312ce3c82afa60ef714 QLSI_2016-04-29_FUS-droplet-780-5.zip
3dc230d213d788d194b053c04ed019104207aa631eac27924215232dce0b0773 QLSI_2016-07-05_PAA-beads-S6-A.cfg
7fa1becd016dcd3bf28c8c4c693e15110d239dca803414eed9eb0a62bc5a9daf QLSI_2016-07-05_PAA-beads-S6-A.zip
ea8143e609217dc54e2ded3185ff4b88605c995376a4b86cb297a44aa4ae7baf SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.cfg
5819ec142b58fd7ef9df614a4ac63c78efdaf9ae9e8f02b5ce2f6215fd9251d1 SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.tif
0b478d422d6510aa4cf8b5d082128c595cac4a4d3b1ce4c53c8b496d410299cf SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.cfg
ab9503b33562968333423b5a541ee41cac135fa1153a835da3c009f9f5c305ed SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.tif
3bb6ed2275f61d03628e5dbea861b7fe87870cd17cd998679cd14da30aaab274 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.cfg
e2b3d79bf3b7904a9184b79746dbeff696f716e6789541ff6835672514a25a93 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.tif


References:

[1] https://dx.doi.org/10.1364/OE.26.010729
[2] https://qpimage.readthedocs.io
[3] https://drymass.readthedocs.io
[4] https://qpformat.readthedocs.io