jp5b02011_si_001.pdf (212.12 kB)
Quantitative Study of the Interfacial Adsorption of Cellullase to Cellulose
journal contribution
posted on 2015-06-25, 00:00 authored by Gang Hu, John A. Heitmann, Biao Zhong, Lucian
A. Lucia, Dimitris
S. ArgyropoulosThe
phenomenon of interfacial adsorption of cellulase to cellulose
plays a significant role in its enzymatic conversion to a variety
of biomaterials and biofuels. The crystallinity and surface areas
are the key substrate characteristics that must be considered in its
final conversion to soluble sugars. This research therefore characterized
the crystallinity and surface areas of microcrystalline celluloses
and hardwood pulps as a function of the interfacial activity of cellulase,
and thereafter the effects of these two parameters on adsorption were
modeled. The crystallinities were characterized by X-ray diffraction,
while surface areas by laser scattering and Congo red adsorption.
It was found that cellulase adsorption to cellulose follows a Langmuir
model while statistical modeling showed that surface area, crystallinity,
and their interactions were determined to be significant for cellulase
adsorption over a temperature range of 4–50 °C.