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Quantitative RT-PCR analysis of imprinted gene expression.

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posted on 2016-05-27, 20:24 authored by Hyun Sik Jang, Yean Ju Hong, Hyun Woo Choi, Hyuk Song, Sung June Byun, Sang Jun Uhm, Han Geuk Seo, Jeong Tae Do

The expression profiles of paternal and maternal imprinted genes were analyzed by real-time RT-PCR. All data are normalized to ACTB expression and calibrated on the ESCs, whose gene expression was considered 1 for all genes. Error bars represent mean values ± SEM of three independent experiments. Student’s t-test: ***, p<0.001; **, p<0.01; *, p<0.05.

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