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Quantitative Analysis of Coenzyme F430 in Environmental Samples: A New Diagnostic Tool for Methanogenesis and Anaerobic Methane Oxidation
journal contribution
posted on 2015-12-17, 01:19 authored by Masanori Kaneko, Yoshinori Takano, Yoshito Chikaraishi, Nanako
O. Ogawa, Susumu Asakawa, Takeshi Watanabe, Seigo Shima, Martin Krüger, Makoto Matsushita, Hiroyuki Kimura, Naohiko OhkouchiCoenzyme F430 is a nickel hydrocorphinoid
and is the prosthetic
group of methyl–coenzyme M reductase that catalyzes the last
step of the methanogenic reaction sequence and its reversed reaction
for anaerobic methane oxidation by ANME. As such, function-specific
compound analysis has the potential to reveal the microbial distribution
and activity associated with methane production and consumption in
natural environments and, in particular, in deep subsurface sediments
where microbiological and geochemical techniques are restricted. Herein,
we report the development of a technique for high-sensitivity analysis
of F430 in environmental samples, including paddy soils, marine sediments,
microbial mats, and an anaerobic groundwater. The lower detection
limit of F430 analysis by liquid chromatography/mass spectrometry
is 0.1 femto mol, which corresponds to 6 × 102 to
1 × 104 cells of methanogens. F430 concentrations
in these natural environmental samples range from 63 × 10–6 to 44 nmol g–1 and are consistent
with the methanogenic archaeal biomass estimated by microbiological
analyses.
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geochemical techniquesF 430paddy soilsQuantitative Analysismarine sedimentsmethane oxidationmethane productionnickel hydrocorphinoidF 430 analysisF 430 concentrationsdetection limit0.1 femto molNew Diagnostic Toolmethanogenic archaeal biomassAnaerobic Methane OxidationCoenzyme F 430ANMEsamples rangeEnvironmental Samplesprosthetic groupmethanogenic reaction sequenceCoenzyme F 430
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