pr5007944_si_001.xlsx (22.18 kB)
Proteomics Unravels Extracellular Vesicles as Carriers of Classical Cytoplasmic Proteins in Candida albicans
dataset
posted on 2015-01-02, 00:00 authored by Ana Gil-Bona, Arancha Llama-Palacios, Claudia Marcela Parra, Fernando Vivanco, César Nombela, Lucía Monteoliva, Concha GilThe
commensal fungus Candida albicans secretes a considerable number of proteins and, as in different
fungal pathogens, extracellular vesicles (EVs) have also been observed.
Our report contains the first proteomic analysis of EVs in C. albicans and a
comparative proteomic
study of the soluble secreted proteins. With this purpose, cell-free
culture supernatants from C. albicans were separated
into EVs and EV-free supernatant and analyzed by LC–MS/MS.
A total of 96 proteins were identified including 75 and 61 proteins
in EVs and EV-free supernatant, respectively. Out of these, 40 proteins
were found in secretome by proteomic analysis for the first time.
The soluble proteins were enriched in cell wall and secreted pathogenesis
related proteins. Interestingly, more than 90% of these EV-free supernatant
proteins were classical secretory proteins with predicted N-terminal
signal peptide, whereas all the leaderless proteins involved in metabolism,
including some moonlighting proteins, or in the exocytosis and endocytosis
process were exclusively cargo of the EVs. We propose a model of the
different mechanisms used by C. albicans secreted
proteins to reach the extracellular medium. Furthermore, we tested
the potential of the Bgl2 protein, identified in vesicles and EV-free
supernatant, to protect against a systemic candidiasis in a murine
model.