pone.0170285.g004.tif (5.87 MB)
Proteomic Analysis of Kveim Reagent Identifies Targets of Cellular Immunity in Sarcoidosis - Fig 4
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posted on 2017-01-23, 17:39 authored by Christian Eberhardt, Muhunthan Thillai, Robert Parker, Nazneen Siddiqui, Lee Potiphar, Rob Goldin, John F. Timms, Athol U. Wells, Onn M. Kon, Melissa Wickremasinghe, Donald Mitchell, Mark E. Weeks, Ajit LalvaniImmunohistochemical staining of spleen tissue shows increased abundance of vimentin in sarcoidosis spleens at 200 x (Fig 4a) and 400 x magnification (Fig 4b) compared to healthy spleen tissue at the same magnifications (Fig 4c and 4d).
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identifier PXD 005150.sarcoidosis spleen tissuealpha-actinin -4. Conclusions Stimulationvimentin stimulation2 D-DIGEsarcoidosis PBMCsMultiplex Cytokine Assaycytokine secretionIFNKv-specific proteinsKveim Reagent Identifiessarcoidosis PBMCs vs1 D-SDS-PAGEpgcontrol PBMCsTNF -α secretionMethods Kv extractsSarcoidosis Background Kveim-reagent74 sarcoidosis tissue-specific proteinspro-inflammatory cytokine secretionsarcoidosis patients vsPeripheral Blood Mononuclear Cells
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