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Proposed CRISPR/Cas systems in genome of YL15.
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posted on 2017-05-19, 17:40 authored by Tangjian Peng, Liyuan Ma, Xue Feng, Jiemeng Tao, Meihua Nan, Yuandong Liu, Jiaokun Li, Li Shen, Xueling Wu, Runlan Yu, Xueduan Liu, Guanzhou Qiu, Weimin ZengThe genome of YL15 harbors (a) a putative type III-B and (b) a putative type I-F CRISPR/Cas system. The type III-B system has a cluster of genes for repeat-associated mysterious proteins (RAMPs, Cmr—Cmr6, with Cmr2/Cas10 as a signature protein), a putative Cas protein and a Cas6 protein which is involved in CRISPR transcript processing. The type I-F CRISPR/Cas system has genes encoding for a Cas1 protein, three csy proteins and a Cas3” protein which has only the HD domain of Cas3 protein. Both of the systems have genes coding for two hypothetical proteins (hyp) with unknown functions.
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RNA transcript countsIII-BbacteriumGenomicstrain YL 15transcriptomicadaptation mechanismse.gUVDNA repair enzymestemperature acid mine drainageacid mine drainagestrategytypesynthesisYulong copper mineGeneI-Fbacterioferritin Dps geneproteinalpine acid mine drainage environmentAcidithiobacillus ferrivorans strain YL 15CRISPRalpine acid mine drainage Acidithiobacillus ferrivoransstressstrain YL 15.analysis
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