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Production of Methionol from 3‑Methylthiopropionaldehyde by Catalysis of the Yeast Alcohol Dehydrogenase Adh4p
journal contribution
posted on 2020-04-09, 15:03 authored by Yixin Che, Sheng Yin, Hui Wang, Huaqing Yang, Ruixin Xu, Qi Wang, Yiping Wu, Julien Boutet, Robert Huet, Chengtao WangMethionol is a sulfur-containing aroma compound that contributes
to the flavors of fermented foods. In this work, a novel method for
methionol production was established using 3-methylthiopropionaldehyde
(MMP) and alcohol dehydrogenase (ADH). First, expression of seven
ADH genes was analyzed in yeast fermentation added with MMP. Only ADH4 displayed an evident increased expression in response
to MMP. ADH4 was then overexpressed in Saccharomyces cerevisiae S288c and Escherichia coli BL21. The recombinant yeast strain
S4 produced more methionol than control strain in MMP fermentation.
Furthermore, 0.55 g/L 42 kDa Adh4p was prepared from E. coli by induced expression and purification. A
fed-batch catalysis system was finally established to produce methionol
from MMP by Adh4p. In 10 h of continuous catalysis, the conversion
rate of MMP remained 80–95%, and a final yield of 0.2 g/L methionol
was achieved. This work proposed a novel method for methionol production
by enzymatic catalysis with a potential application prospect in industry.