Pregnane glycoside from <i>Huernia saudi-arabica</i> as latent schistosomicidal mediator

<p>Development of a novel agent for control of schistosomiasis is a mandate. <i>In-vitro</i> anti-schistosomal activity of the aerial parts of <i>Huernia saudi-arabica</i> were examined. Chromatographic investigations of the ethanol extract (EE) were afforded three compounds. Pregnane glycoside (CI) 12-<i>β-p</i>-hydroxy-benzoyl-20-<i>O-</i>acetyl-boucerin-3-<i>O-β</i>-D-glucopyranosyl-(1→4)-<i>β</i>-D-cymaropyranosyl-(1→4)-<i>β-</i>D-cymaropyranoside, in addition to two flavonoids (CII) luteolin-4'-<i>O</i>-<i>β</i>-D-neohesperidoside and (CIII)quercetin-3-rutinoside were recognized <i>via</i> spectral analysis. The schistosomicidal effects were evaluated using scanning electron microscope (SEM). <i>In-vitro</i> bioassays on the viability (mobility, morphological changes and mortality) of <i>Schistosoma mansoni</i> adults, cercariae, miracidia and eggs at different concentrations 2.5, 5, 12.5, 25 and 50 μg/ml of EE and 2.6, 5.2, 13, 26 and 52 μM of CI in incubation times 1,2,4,6,12hrs were carried out. EE and CI evidenced <i>in-vitro</i> anti-schistosomal activity with a dose and incubation time-dependent fashion. The effect of EE and CI was evident by the topography damage showed by SEM. EE proved moderate <i>in-vitro</i> cytotoxicity with IC<sub>50</sub> of 8.48 µg/ml.</p>