PrP^C abundance is not limiting for low conversion efficiency prion strains in PMCAsi.

Epitope accessibility immunoassay measurement of the fold change of PrP^C (blue squares) and PrP^Sc (red circles) after one round of PMCA or PMCAsi from reactions seeded with either uninfected (UN), HY-infected, DY-infected, 139H-infected, ME7H-infected, or co-infected with DY and 139H scrapie-infected, or DY and ME7H-infected brain homogenates. Error bars represent SEM. The experiment was repeated a minimum of three times with similar results.