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Potentiometric Adsorption Isotherm Analysis of a Molecularly Imprinted Polymer Interface for Small-Biomolecule Recognition
Version 2 2018-05-18, 18:42
Version 1 2018-05-18, 17:34
journal contribution
posted on 2018-05-18, 18:42 authored by Shoichi Nishitani, Toshiya SakataIn
this paper, we report a direct and quantitative analytical method
of small-biomolecule recognition with a molecularly imprinted polymer
(MIP) interface, taking advantage of the potentiometric principle
of a field-effect transistor (FET) sensor, which enables the direct
detection of ionic charges without using labeling materials such as
fluorescent dyes. The interaction of low-molecular-weight oligosaccharides
such as paromomycin and kanamycin with the MIP interface including
phenylboronic acid (PBA) was directly and quantitatively analyzed
from the electrical signals of an MIP-coated FET sensor. In particular,
the change in the potential response of the FET sensor was derived
on the basis of the multi-Langmuir adsorption isotherm equations,
considering the change in the molecular charges of PBA caused by the
adsorption equilibrium of the analytes with the vinyl PBA-copolymerized
MIP membrane. Thus, the potentiometric adsorption isotherm analysis
can elucidate the formation of selective binding sites at the MIP
interface. The electrochemical analysis of the functional biointerface
used in this study supports the design and construction of sensors
for small biomarkers.