bm9b00641_si_001.pdf (1.08 MB)
Polydiacetylene (PDA) Liposome-Based Immunosensor for the Detection of Exosomes
journal contribution
posted on 2019-08-06, 12:34 authored by Changheon Kim, Kangwon LeeExosomes
are extracellular vesicles (EVs) that have attracted attention
because of their important biological roles in intercellular communication
and transportation of various biomolecules, including proteins and
genetic materials. However, due to difficulties in their selective
capture and detection, further application of exosomes remains challenging.
To detect EVs, we fabricated a liposomal biosensor based on polydiacetylene
(PDA), a conjugate polymer that has been widely used in sensing applications
derived from its unique optical properties. To confer selectivity
and sensitivity to the sensory material, antibodies targeting CD63,
a membrane protein exclusively found in exosomes, were attached to
the PDA liposomes and phospholipid molecules were incorporated into
the PDA vesicles. Signal analysis derived from PDA liposomes for exosome
detection and quantification was performed by observing colorimetric
changes triggered by the ligand–receptor interaction of PDA
vesicles. Visual, UV–visible, and fluorescence spectroscopic
methods were used to obtain signals from the PDA lipid immunosensor,
which achieved a detection limit of 3 × 108 vesicles/mL,
the minimum concentration that can be used in practical applications.
The strategies used in the system have the potential to expand into
the field of dealing with exosomes.