bm5b00900_si_001.pdf (1.25 MB)
Polyamide Nanogels from Generally Recognized as Safe Components and Their Toxicity in Mouse Preimplantation Embryos
journal contribution
posted on 2015-11-09, 00:00 authored by Priyaa Prasad, Mijanur
Rahaman Molla, Wei Cui, Mine Canakci, Barbara Osborne, Jesse Mager, S. ThayumanavanSafe delivery systems that can not
only encapsulate hydrophobic
drug molecules, but also release them in response to specific triggers
are important in several therapeutic and biomedical applications.
In this paper, we have designed a nanogel based on molecules that
are generally recognized as safe (GRAS). We have shown that the resultant
polymeric nanogels exhibit responsive molecular release and also show
high in vitro cellular viability on HEK 293T, HeLa,
MCF 7, and A549 cell lines. The toxicity of these nanogels was further
evaluated with a highly sensitive assay using mouse preimplantation
embryo development, where blastocysts were formed after 4 days of in vitro culture, and live pups were born when morulae/early
blastocysts were transferred to the uteri of surrogate recipients.
Our results indicate that these nanogels are nontoxic during mammalian
development and do not alter normal growth or early embryo success
rate.