Pico Green staining and flow cytometry analysis of P. aeruginosa PAO1 in water.

Log phase cultures of PAO1 were inoculated into water for incubation. Each sample was stained with Quant-iT PicoGreen and subjected to flow cytometry to analyze the dsDNA content in cells during long-term incubation in water. Cells from mid-log PAO1 cultures were compared to cells in water at day 0, 3 weeks and 4 weeks. Each panel represents a population of 50,000 cells per experiment. This figure shows a representation of one of the three trials performed and demonstrates the increased fluorescence and DNA staining observed over time in water.