Phosphate leak in model with PPP.

Time course simulation of model B, in which the reactions of the glycosomal PPP are switched on at t = 0 by increasing their V_max value from zero to the value given in Table S1. Glc_e is 5 mM and k_TOX = 2 µl·min⁻¹·mg protein⁻¹. Solid lines indicate medians, shaded areas show interquartile ranges. Fluxes (J) are plotted on the left y-axis and are indicative of glucose uptake (GlcT_{plasma membrane}), glycerol (GK) and pyruvate production (PyrT) and the two branches of pentose phosphate pathways (G6PDH_c/g). The sum of bound phosphates in the glycosome (ΣP_g), as exists in the model of glycolysis (Table 2, moiety 5), is plotted on the right y-axis. Within 25 minutes, all bound phosphates within the glycosome are depleted and all metabolic fluxes subsequently drop to zero.