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Phenotype of cells collected after immature dendritic cell preparation procedure in cancer patients

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posted on 2011-12-31, 00:01 authored by Alban Gervais, Jean Levêque, Françoise Bouet-Toussaint, Florence Burtin, Thierry Lesimple, Laurent Sulpice, Jean-Jacques Patard, Noelle Genetet, Véronique Catros-Quemener

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Taken from "Dendritic cells are defective in breast cancer patients: a potential role for polyamine in this immunodeficiency"

Breast Cancer Research 2005;7(3):R326-R335.

Published online 25 Feb 2005

PMCID:PMC1143555.

Copyright © 2005 Gervais et al.; licensee BioMed Central Ltd

Peripheral blood mononuclear cells from healthy donors (= 11) or patients with colorectal cancer (= 7), renal cell carcinoma (= 10), or breast cancer (= 15) were depleted of lymphocytes (2-hour adherence step) and cultured for 7 days in the presence of granulocyte–macrophage colony-stimulating factor and IL-4. Data are expressed as the percentage of cells (with standard error) expressing the HLA-DRLinand HLA-DRLinphenotype. *< 0.01 versus healthy donors.

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