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Perturbations in host ribosome or global translation lead to increased Wolbachia levels in the fly.

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posted on 2018-11-13, 18:33 authored by Yolande Grobler, Chi Y. Yun, David J. Kahler, Casey M. Bergman, Hangnoh Lee, Brian Oliver, Ruth Lehmann

(A, C)Wolbachia-infected ovarioles of control (Sp/CyO) and ribosome mutant (RpL27A1/CyO) siblings processed to visualize Wolbachia using a 23s rRNA FISH probe (magenta) and cell nuclei (DAPI) in early stages including the germaria (A) and later stages including stage 10 Drosophila egg chambers (C). Scale bars represent 33μm. (B, D) Quantification of integrated density of the Wolbachia FISH probe in germaria (B) and stage 10 egg chambers (D) collected from 15–25 Drosophila ovary pairs for each genotype. Bars represent median. Differences between control and mutant siblings is statistically significant (Non-parametric, Mann Whitney). (E) Wolbachia-infected Drosophila flies fed on cycloheximide-containing food or control food for 7 days. Cycloheximide-fed flies displayed significantly increased Wolbachia levels in the whole fly as measured by DNA qPCR (Mann Whitney, p<0.001). Results displayed as fold-change relative to control food fed flies. Each data point represents an individual fly. Bars represent mean fold change. See S10-14 for further results in the fly for ribosomal and proteasomal mutants.

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