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Peptidomic Analyses of Mouse Astrocytic Cell Lines and Rat Primary Cultured Astrocytes
journal contribution
posted on 2012-08-03, 00:00 authored by Ping Yin, Ann M. Knolhoff, Harry
J. Rosenberg, Larry J. Millet, Martha U. Gillette, Jonathan V. SweedlerAstrocytes play an active role in the modulation of synaptic
transmission by releasing cell–cell signaling molecules in
response to various stimuli that evoke a Ca2+ increase.
We expand on recent studies of astrocyte intracellular and secreted
proteins by examining the astrocyte peptidome in mouse astrocytic
cell lines and rat primary cultured astrocytes, as well as those peptides
secreted from mouse astrocytic cell lines in response to Ca2+-dependent stimulations. We identified 57 peptides derived from 24
proteins with LC–MS/MS and CE–MS/MS in the astrocytes.
Among the secreted peptides, four peptides derived from elongation
factor 1, macrophage migration inhibitory factor, peroxiredoxin-5,
and galectin-1 were putatively identified by mass-matching to peptides
confirmed to be found in astrocytes. Other peptides in the secretion
study were mass-matched to those found in prior peptidomics analyses
on mouse brain tissue. Complex peptide profiles were observed after
stimulation, suggesting that astrocytes are actively involved in peptide
secretion. Twenty-six peptides were observed in multiple stimulation
experiments but not in controls and thus appear to be released in
a Ca2+-dependent manner. These results can be used in future
investigations to better understand stimulus-dependent mechanisms
of astrocyte peptide secretion.
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Cultured AstrocytesAstrocytesOther peptidespeptidomics analysesPeptidomic Analysessynaptic transmissionfuture investigations57 peptidesMouse Astrocytic Cell Linesastrocyte peptide secretionstimulation experimentsastrocyte intracellularastrocyte peptidomeCEsecretion studymouse brain tissueLC24 proteinspeptide secretionComplex peptide profilesmouse astrocytic cell lines
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