Peptidoglycan–polysaccharide (PG-PS)-induced activation of nuclear factor-κB (NF-κB) is inhibited in CD14 knockout spleen macrophages

<p><b>Copyright information:</b></p><p>Taken from "CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls"</p><p>Arthritis Research & Therapy 2004;6(3):R273-R281.</p><p>Published online 27 Apr 2004</p><p>PMCID:PMC416450.</p><p>Copyright © 2004 Li et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> Spleen macrophages were stimulated for 30 min with PG-PS at a concentration of 20 μg/ml. The left column shows immunostaining of the p65 (RelA) subunit of NF-κB, and the right column shows counterstaining of nuclei with Hoechst 33342. The rows show the following: top – unstimulated cells from wild-type mice; middle – PG-PS stimulated cells from wild-type mice; and bottom row – PG-PS-stimulated cells from CD14 knockout mice. Representative data from three independent experiments are shown.