Parameter choices do not qualitatively change the biological interpretation of our information entropy measure.

<p>Cohen’s d statistic [<a href="" target="_blank">29</a>] comparing distributions of entropy values for cellular calcium activity of (a) stage 14 and stage 18 <i>Xenopus laevis</i> embryos, (b) stage 18 and stage 22 <i>Xenopus laevis</i> embryos, (c) stage 14 and stage 22 <i>Xenopus laevis</i> embryos, and (d) mature retrotrapezoid nucleus neurons from embryonic mice in pH 7.4 solution vs. pH 7.2 solution (data in (d) obtained from [<a href="" target="_blank">15</a>]). At large values of <i>n</i> and <i>k</i>, a sign change in d value occurs which is a technical artifact arising from there being more entries in the transition matrix than can be filled by data from our time series. The numerical values of d which generated this figure can be found in <a href="" target="_blank">S2 Table</a>.</p>