Parallel, Large-Scale, and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-Length Sequence by Polymerization Technique

The catching by polymerization synthetic oligodeoxynucleotide (ODN) purification technique was shown to be potentially suitable for high-throughput purification by the purification of 12 ODNs simultaneously, to be convenient for large-scale purification by purification at the 60 μmol synthesis scale, and to be highly powerful for purification of long ODNs by purification of ODNs as long as 303-mers. LC–MS analysis indicated that the ODNs purified with the technique have excellent purity.