PM induces AJ breakdown.
(A) Cells were treated with PM (20 μg/cm2, 1h) and cell junctions were visualized by immunostaining with VE-cadherin antibody. (B) Surface protein biotinylation assay was performed as described in Methods and lysates were run on Western blot to detect biotinylated and total VE-cadherin. (C) Membrane fractions isolated by sub-cellular protein fractionations were subjected for Western blotting against p120-catenin and VE-cadherin antibodies. The total cell lysates were used as normalization control.