pone.0206251.g001.tif (887.07 kB)
PM causes endothelial barrier disruption.
figure
posted on 2018-11-12, 18:40 authored by Pratap Karki, Angelo Meliton, Alok Shah, Yufeng Tian, Tomomi Ohmura, Nicolene Sarich, Anna A. Birukova, Konstantin G. Birukov(A) Human pulmonary lung EC were exposed to indicated concentrations of PM and TER was measured across the cell monolayers over time. (B) Cells grown on immobilized biotinylated gelatin were exposed to PM for 4 hr and FITC-avidin (25 μg/mL) was added for 3 min. After washing unbound FITC-avidin with PBS, FITC fluorescence was determined in Victor X5 plate reader. Normalized readings are expressed as mean ± S.D.; n = 6, *p<0.05. (C) Visualization of FITC fluorescence in control or PM-treated cells (20 μg/cm2, 4 h).
