figshare
Browse
ppat.1006648.g008.tif (292.02 kB)

PKA negatively regulates innate antiviral response in mice.

Download (292.02 kB)
figure
posted on 2017-09-21, 18:23 authored by Bing-Ru Yan, Lu Zhou, Ming-Ming Hu, Mi Li, Heng Lin, Yan Yang, Yan-Yi Wang, Hong-Bing Shu

(A) Knockdown of PKACs inhibits SeV- and EMCV-induced transcription of downstream antiviral genes in BMDCs. BMDCs were transfected with PKACs siRNA for 36 h, then infected with SeV (MOI = 1) or EMCV (MOI = 1) for the indicated times before qPCR analysis. (B) H89 potentiates EMCV-induced serum cytokine levels in mice. Mice (n = 6) were infected i.p. with EMCV at 1×107 pfu per mouse for 12 h before serum cytokines were measured by ELISA. (C) H89 inhibits EMCV-induced death in mice. Mice (n = 8) were infected i.p. with EMCV at 1×105 pfu per mouse, and the survival rates of mice were observed and recorded for 2 weeks.

History