PCR analysis of tag-<i>UBC13</i>/<i>RAD5</i> yeast strains.

<p>(A) mCherry-Ubc13 strain. The left panel displays the genomic DNA PCR data. Lane 1: DNA ladder marker. Lane 2: the original strain HK578-10A. Lane 3: the pop-in strain CUC-Ubc13. Lane 4: the pop-out strain mCherry-Ubc13. The right panel represents the corresponding genomic organizations at the locus of <i>UBC13</i> in these three different strains. (B) Rad5-sfGFP strain. The original strain is HK578-10D. (C) Rad5-mKikGR strain. The original strain is HK578-10D. Arrows indicate the locations of primers. The detail information of primers could be found in the part of yeast strains check primers in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0176184#pone.0176184.s006" target="_blank">S3 Table</a>.</p>