PCR Sprint Programmed T vs Recorded T

2012-08-23T18:51:49Z (GMT) by Anthony Salvagno
<p>I put the machine in manual mode so I can change the T when I needed to and I would take data points at 30 seconds for melting and annealing temperatures and at 30s and 5 minutes for extending T. This is how long I would be doing that step during the PCR reaction so it seemed to make sense. Attached is a chart of the Set T and the recorded T at each interval, the raw data of recorded T, a converted recorded T excel file, and the plot of that data.</p>



CC BY 4.0