PCR-based alternative for diagnosis of immunoglobulin heavy chain gene rearrangement: principles, practice, and polemics

2012-11-17T20:12:56Z (GMT) by Salvador J. Diaz-Cano
<p>PCR-based detection of immunoglobulin gene rearrangements is a reliable and accurate method which can be utilized as a clinical test if appropriate quality controls are performed (internal, polyclonal and monoclonal, sensitivity and specificity controls) and strict criteria for clonal rearrangements are used to exclude false-positives and false-negatives.</p>