Oxidative stress protective effect of <i>Dracocephalum multicaule</i> essential oil against human cancer cell line

<div><p>In this study, we report the antioxidative and protective effect of essential oil of <i>Dracocephalum multicaule</i> on K562 cells. Our results demonstrated that monoterpenoids, including oxygenated and hydrocarbons, 71.5% and 28.3%, respectively, were the principal essential oils of <i>D. multicaule</i>. Perilla aldehyde (71.5%) and limonene (28.1%) were identified as the main components. Antioxidant studies based on the 2,2-diphenylpicrylhydrazyl assay indicated that the <i>D. multicaule</i> essential oil possesses a marked antioxidant and radical-scavenging activity with an IC<sub>50</sub> value of 438.2 μg/mL. Pretreatment with essential oil and main constituents protected K562 cells 49.5% against H<sub>2</sub>O<sub>2</sub>-induced oxidative damage throughout increasing the activities of antioxidant enzymes and glutathione content in K562 cells. Collectively, <i>D. multicaule</i> essential oil and its main compounds especially in combinatory condition at a ratio of 7:3 with high antioxidant properties may be able to protect cells against oxidative stress induced by H<sub>2</sub>O<sub>2</sub> through antioxidative mechanisms.</p></div>