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Overgrowth caused by loss of Tsc1 is dependent on TORC1 signaling.

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posted on 2018-04-20, 17:35 authored by Katarzyna Nowak, Avantika Gupta, Hugo Stocker

(A) Western blot analysis of proteins extracted from the control or Tsc1 mutant eye imaginal discs. The extremely small size of the control eye discs from animals reared on NR precluded the Western analysis. Total levels of S6K were unaltered. β-tubulin served as loading control. (B) Eyes with eyFlp control, Tsc1, Rheb, and combination of Tsc1 and Rheb clones (marked by the absence of pigmentation) of animals reared under normal food and NR; (B’) quantification of eye size. (C) Eye discs bearing MARCM control and Tsc1 mutant clones, with or without the knockdown of Raptor or S6K (marked by GFP) dissected from larvae reared on normal food or NR. Scale bars are 250 μm.

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