Overexpression of GPATCH3 inhibits RLR-mediated signaling.
(A) 293T cells (1 x 105) were cotransfected with empty vectors or increased amounts of GPATCH3 expression plasmids together with indicated luciferase reporters (0.05 μg each). Twenty-four hours after transfection, cells were stimulated with SeV for 12 hours before luciferase assays were performed. (B) The experiments were similarly performed as in A, except that cells were stimulated with TNF α (20 ng/ml). Graphs show mean ± SD. n = 3. *P<0.05, **P<0.01 (Student’s t-test).