Overexpressing GBP1 and GBP2 in the fat body rescues body size in <i>gbp1</i>, <i>gbp2 ex67</i> null mutant larvae.

2016-02-29T07:36:38Z (GMT) by Takashi Koyama Christen K. Mirth
<p><b>(A, B)</b> Overexpressing both GBP1 and GBP2 partially rescues body size reduction in <i>gbp1</i>, <i>gbp2 ex67</i> mutant females (A) and males (B) at 22°C. <i>n</i> = 51–52 for A and <i>n</i> = 54–65 for B. <b>(C)</b> Overexpressing both GBP1 and GBP2 partially increases growth rate in <i>gbp1</i>, <i>gbp2 ex67</i> mutant larvae. <i>n</i> = 14–20/time point. <b>(D)</b> Overexpressing both GBP1 and GBP2 partially rescues the duration of the L3 in <i>gbp1</i>, <i>gbp2 ex67</i> mutant larvae. <i>n</i> = 111–119. <b>(E, F)</b> Overexpressing both GBP1 and GBP2 reduces ILP2 (E) and ILP5 (F) accumulation in the insulin-producing cells of <i>gbp1</i>, <i>gbp2 ex67</i> mutant larvae. Larvae were staged at the onset of the L3, and then fed on normal food for 24 h. The insulin-producing cells were immunostained using an anti-ILP2 antibody and an anti-ILP5 antibody. <b>(G, H)</b> Overexpressing both GBP1 and GBP2 reduces the densities of ILP2 (G) and ILP5 (H) signals in the insulin-producing cells. The densities of ILP2 and ILP5 were quantified using ImageJ. We standardized the densities of ILPs by fixing the values from <i>C7</i>>GFP in the <i>gbp1</i>, <i>gbp2 ex67</i> mutant background to 1. <i>n</i> = 30–60. <b>(I)</b> Overexpressing both GBP1 and GBP2 reduces FRE-luciferase activity in the entire body of <i>gbp1</i>, <i>gbp2 ex67</i> mutant larvae. <i>n</i> = 5. Two copies of UAS transgenes were expressed using the <i>C7</i> Gal4 driver. For the wild-type control, we overexpressed two copies of UAS GFP using the <i>C7</i> Gal4 driver. Treatments sharing the same letter indicate the groups that are statistically indistinguishable from one another (ANOVA and pairwise <i>t</i> tests, <i>p</i> < 0.05). Growth rate was analyzed by ANCOVA and post hoc comparisons of the slopes. The supplementary file in which the data used to generate each plot can be found is <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002392#pbio.1002392.s001" target="_blank">S1 Data</a>.</p>