Other Pex mutants show a phenotype similar to Pex19 mutants and can also be rescued with coconut oil.

(A) Survival of different Pex mutants on control and rescue diet. n = 5 in groups of 25 individuals. (B-I) TMRE staining of third-instar larval Malpighian tubules. Scale bars represent 10 μm. n = 5. (J) FAMEs comparing w- and Pex mutants fed with control and M/LCFA-enriched rescue food. n = 3. Genotypes are Pex2^HP35039/Pex2^f01899, Pex3²/ Df(32)6262, Pex5^MI06050/ Pex5^MI06050, Pex10^MI04076/ Pex10^MI04076, Pex19^ΔF7/ Df(2L)esc ^p3-0, Pex19^ΔF7/ Pex19^ΔF7. Error bars represent SD. Asterisks represent *p < 0.05, **p < 0.01, ***p < 0.001. Significance tested using ANOVA with Tukey posttest. Corresponding raw data can be found in supplemental file S1 Data. FAME, fatty acid methyl ester; M/LCFA, medium- and long-chain fatty acid; NEFA, nonesterified fatty acid; Pex, peroxin; TMRE, tetramethylrhodamine ethyl ester.