OA modulates ASI neuropeptide release.

Animals expressing ASI::nlp-9::gfp transgenes in their respective null background were incubated with either TA or OA and analyzed for GFP fluorescence. (A) ASI::nlp-9::gfp in nlp-9 null animals. (B) Axonal analysis of ASI::nlp-9::gfp fluorescence in extra-synaptic/synaptic sites and right/left ASIs. The gpa-4 promoter was used to drive ASI expression. All monoamine concentrations were 10 mM. * Denotes significantly different from control (p ≤ 0.05). Data are presented as a mean ± SE (n) and were analyzed by One-way ANOVA (5A n = 38–55 One-way ANOVA: Total p = 0.0001, Dunnett T3 = p 0.028, Axon p = 0.0001, Dunnett t (2-sided) = 0.004. 5B n = 20–55 (L = 20–28, R = 18–28), One-way ANOVA: Synaptic p = 0.0001, Dunnett t (2-sided) = p 0.003, ASIR p = 0.0001, Dunnett T3 p = 0.0001).