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Nuclease-Functionalized Poly(Styrene‑b‑isobutylene‑b‑styrene) Surface with Anti-Infection and Tissue Integration Bifunctions
journal contribution
posted on 2014-10-22, 00:00 authored by Shuaishuai Yuan, Jie Zhao, Shifang Luan, Shunjie Yan, Wanling Zheng, Jinghua YinHydrophobic thermoplastic elastomers,
e.g., poly(styrene-b-isobutylene-b-styrene) (SIBS), have found
various in vivo biomedical applications. It has long been recognized
that biomaterials can be adversely affected by bacterial contamination
and clinical infection. However, inhibiting bacterial colonization
while simultaneously preserving or enhancing tissue-cell/material
interactions is a great challenge. Herein, SIBS substrates were functionalized
with nucleases under mild conditions, through polycarboxylate grafts
as intermediate. It was demonstrated that the nuclease-modified SIBS
could effectively prevent bacterial adhesion and biofilm formation.
Cell adhesion assays confirmed that nuclease coatings generally had
no negative effects on L929 cell adhesion, compared with the virgin
SIBS reference. Therefore, the as-reported nuclease coating may present
a promising approach to inhibit bacterial infection, while preserving
tissue-cell integration on polymeric biomaterials.