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Novel Platform of Cardiomyocyte Culture and Coculture via Fibroblast-Derived Matrix-Coupled Aligned Electrospun Nanofiber
journal contribution
posted on 2016-12-12, 00:00 authored by Muhammad Suhaeri, Ramesh Subbiah, Su-Hyun Kim, Chong-Hyun Kim, Seung Ja Oh, Sang-Heon Kim, Kwideok ParkFor
cardiac tissue engineering, much attention has been given to the artificial
cardiac microenvironment in which anisotropic design of scaffold and
extracellular matrix (ECM) are the major cues. Here we propose poly(l-lactide-co-caprolactone) and fibroblast-derived
ECM (PLCL/FDM), a hybrid scaffold that combines aligned electrospun
PLCL fibers and FDM. Fibroblasts were grown on the PLCL fibers for
5–7 days and subsequently decellularized to produce PLCL/FDM.
Various analyses confirmed aligned, FDM-deposited PLCL fibers. Compared
to fibronectin (FN)-coated electrospun PLCL fibers (control), H9c2
cardiomyoblast differentiation was significantly effective, and neonatal
rat cardiomyocyte (CM) phenotype and maturation was improved on PLCL/FDM.
Moreover, a coculture platform was created using multilayer PLCL/FDM
in which two different cells make indirect or direct cell–cell
contacts. Such coculture platforms demonstrate their feasibility in
terms of higher cell viability, efficiency of target cell harvest
(>95% in noncontact; 85% in contact mode), and molecular diffusion
through the PLCL/FDM layer. Coculture of primary CMs and fibroblasts
exhibited much better CM phenotype and improvement of CM maturity
upon either direct or indirect interactions, compared to the conventional
coculture systems (transwell insert and tissue culture plate (TCP)).
Taken together, our platform should be very useful and have significant
contributions in investigating some scientific or practical issues
of crosstalks between multiple cell types.
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Keywords
FDM-deposited PLCL fiberstissue engineeringTCPfibroblast-derived ECMSuch coculture platformsNovel PlatformCM phenotypePLCL fibersFNtissue culture plateCardiomyocyte Culturecoculture systemsrat cardiomyocyteFibroblast-Derived Matrix-Coupled Aligned Electrospun Nanofibercell viabilityelectrospun PLCL fiberscell typesextracellular matrixCM maturitycoculture platformanisotropic design
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