Nodal signaling is required for closure of the anterior neural tube in zebrafish-9
2011-12-31T03:53:45Z (GMT) by
Taken from "Nodal signaling is required for closure of the anterior neural tube in zebrafish"
BMC Developmental Biology 2007;7():126-126.
Published online 8 Nov 2007
PMCID:PMC2214732.siblings carrying the :eGFP transgene, which expresses GFP throughout the pineal anlage during embryogenesis . All mutants used in this experiment had cyclopic eyes. (A-D) Composite bright field and fluorescent images at 2 dpf showing fluorescence in the pineal anlage of embryos having (A-B) normal, round-shaped pineal anlagen, and (C) elongated and (D) divided pineal anlagen. (E-H") Each row shows the pineal of an individual embryo over three days of development as assayed by fluorescence microscopy on a compound microscope. Note the similarity in the morphology of the pineal in (E-E") the WT embryo and (F-F") one of the mutants. In contrast, the mutants in G-G" and H-H" maintain their abnormal elongated and divided morphologies throughout the experiment. All images are dorsal views with anterior to top. Scale bars: 60 μm (A-D), 30 μm (E-H").