ac9b03383_si_001.pdf (725.44 kB)
Nitrobenzoxadiazole Ether-Based Near-Infrared Fluorescent Probe with Unexpected High Selectivity for H2S Imaging in Living Cells and Mice
journal contribution
posted on 2019-10-03, 18:33 authored by Shengyi Gong, Enbo Zhou, Jiaxin Hong, Guoqiang FengH2S is an important endogenous gasotransmitter, and
its detection in living systems is of great significance. Especially,
selective and sensitive near-infrared (NIR) fluorescent H2S probes with rapid response and large Stokes shift are highly desirable
because of their superiority for in vivo detection. Probes with nitrobenzoxadiazole
(NBD) ether as reaction sites have been well-explored recently to
detect biothiols or H2S/biothiols simultaneously, rather
than to detect H2S selectively. In this work, a new NBD
ether-based NIR fluorescent probe was developed, which was unexpectedly
found to show high selectivity for H2S over various other
analytes including biothiols, making it practical for specific detection
of H2S both in vitro and in vivo. Upon response to H2S, this probe showed rapid and significant turn-on NIR emission
changes centered at 744 nm within 3 min, together with a remarkable
large Stokes shift (166 nm) and high sensitivity (LOD: 26 nM). Moreover,
imaging exogenous and endogenous H2S in living cells and
rapid imaging of H2S in living mice with this probe was
successfully applied with excellent performance.