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Ninhydrin detection of amino group containing lipids of PAO1 cells following incubation in water.

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posted on 2018-09-20, 20:10 authored by Shawn Lewenza, Jason Abboud, Karen Poon, Madison Kobryn, Istvan Humplik, John Rainer Bell, Laura Mardan, Shauna Reckseidler-Zenteno

Total lipids were extracted, separated by thin layer chromatography, and sprayed with ninhydrin to visualize the amino group-containing lipids. Lipid samples of water cultures at day 0, day 7 and day 14 were run on TLC plate, alongside controls of lipids extracted from cultures grown in BM2-defined media with limiting (400 μM) and high phosphate (1.6 mM) conditions. The positions of the primary membrane lipid phosphatidylethanolamine (PE) and the unique ornithine lipid (OL) species that is produced under phosphate limitation [15] are indicated.

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