New sesquiterpene and thiophene derivatives from <i>Artemisia rupestris</i>

<div><p></p><p>A new sesquiterpene, rupestrisin A (<b>1</b>), and three new thiophene derivatives, rupestrienes A–C (<b>2</b>–<b>4</b>), were isolated from <i>Artemisia rupestris</i>. Their structures were determined by analyses of MS and NMR spectroscopic data, and the absolute configuration of <b>1</b> was established by calculated ECD spectra using time-dependent density functional theory. In <i>in vitro</i> bioassays, compounds <b>1</b>–<b>4</b> showed inhibitory effects on LPS-stimulated NO production in BV-2 microglial cells with IC<sub>50</sub> values of 24.3, 20.3, 8.5, and 5.3 μM, respectively.</p></div>