New gene discovery plot for pan-genome analysis of 21 Mannheimia haemolytica genomes.

Bars represent the number of new genes as the function of the number n or strains sequentially added. The curve represents the least-squares fit for the function y = AxB with the best fit obtained with a correlation r2 = 0.875 for A = 566.17 ± 3.16, B = -0.22. The extrapolated number of new genes expected after the number of genomes increases to 100 is 123 new genes.