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Nanotubes-Embedded Indocyanine Green–Hyaluronic Acid Nanoparticles for Photoacoustic-Imaging-Guided Phototherapy
journal contribution
posted on 2016-02-10, 00:00 authored by Guohao Wang, Fan Zhang, Rui Tian, Liwen Zhang, Guifeng Fu, Lily Yang, Lei ZhuPhototherapy is a light-triggered
treatment for tumor ablation and growth inhibition via photodynamic
therapy (PDT) and photothermal therapy (PTT). Despite extensive studies
in this area, a major challenge is the lack of selective and effective
phototherapy agents that can specifically accumulate in tumors to
reach a therapeutic concentration. Although recent attempts have produced
photosensitizers complexed with photothermal nanomaterials, the tedious
preparation steps and poor tumor efficiency of therapy still hampers
the broad utilization of these nanocarriers. Herein, we developed
a CD44 targeted photoacoustic (PA) nanophototherapy agent by conjugating
Indocyanine Green (ICG) to hyaluronic acid nanoparticles (HANPs) encapsulated
with single-walled carbon nanotubes (SWCNTs), resulting in a theranostic
nanocomplex of ICG-HANP/SWCNTs (IHANPT). We fully characterized its
physical features as well as PA imaging and photothermal and photodynamic
therapy properties in vitro and in vivo. Systemic delivery of IHANPT theranostic nanoparticles led to the
accumulation of the targeted nanoparticles in tumors in a human cancer
xenograft model in nude mice. PA imaging confirmed targeted delivery
of the IHANPT nanoparticles into tumors (T/M ratio = 5.19 ± 0.3).
The effect of phototherapy was demonstrated by low-power laser irradiation
(808 nm, 0.8 W/cm2) to induce efficient photodynamic effect
from ICG dye. The photothermal effect from the ICG and SWCNTs rapidly
raised the tumor temperature to 55.4 ± 1.8 °C. As the result,
significant tumor growth inhibition and marked induction of tumor
cell death and necrosis were observed in the tumors in the tumors.
There were no apparent systemic and local toxic effects found in the
mice. The dynamic thermal stability of IHANPT was studied to ensure
that PTT does not affect ICG-dependent PDT in phototherapy. Therefore,
our results highlight imaging property and therapeutic effect of the
novel IHANPT theranostic nanoparticle for CD44 targeted and PA image-guided
dual PTT and PDT cancer therapy.