ja4128949_si_001.pdf (1.06 MB)
Nanomolar Pyrophosphate Detection in Water and in a Self-Assembled Hydrogel of a Simple Terpyridine-Zn2+ Complex
journal contribution
posted on 2014-04-16, 00:00 authored by Sandip Bhowmik, Biswa Nath Ghosh, Varpu Marjomäki, Kari RissanenA simple
terpyridine-Zn(II) complex is shown to act as an efficient
and highly selective fluorescent sensor for pyrophosphate in water
at physiological pH. The sensor complex showed an unprecedented fluorescence
response (∼500 fold increase) and a record nanomolar sensitivity
(detectable fluorescent response at 20 nM and LOD ∼ 0.8 nM).
It has successfully been used to stain and record confocal fluorescence
microscopy images of HeLa cells. Moreover, the complex was found to
self-assemble into a hydrogel which was subsequently used to coat
disposable paper strips for easy, low-cost detection of pyrophosphate.