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N‑Linked Glycan Profiling in Neuroblastoma Cell Lines
journal contribution
posted on 2015-05-01, 00:00 authored by Yunli Hu, Anoop Mayampurath, Saira Khan, Joanna
K. Cohen, Yehia Mechref, Samuel L. VolchenboumAlthough MYCN amplification
has been associated
with aggressive neuroblastoma, the molecular mechanisms that differentiate
low-risk, MYCN-nonamplified neuroblastoma from high-risk, MYCN-amplified disease are largely unknown. Genomic and
proteomic studies have been limited in discerning differences in signaling
pathways that account for this heterogeneity. N-Linked glycosylation
is a common protein modification resulting from the attachment of
sugars to protein residues and is important in cell signaling and
immune response. Aberrant N-linked glycosylation has been routinely
linked to various cancers. In particular, glycomic markers have often
proven to be useful in distinguishing cancers from precancerous conditions.
Here, we perform a systematic comparison of N-linked glycomic variation
between MYCN-nonamplified SY5Y and MYCN-amplified NLF cell lines with the aim of identifying changes in
sugar abundance linked to high-risk neuroblastoma. Through a combination
of liquid chromatography–mass spectrometry and bioinformatics
analysis, we identified 16 glycans that show a statistically significant
change in abundance between NLF and SY5Y samples. Closer examination
revealed the preference for larger (in terms of total monosaccharide
count) and more sialylated glycan structures in the MYCN-amplified samples in comparison to smaller, nonsialylated glycans
that are more dominant in the MYCN-nonamplified samples.
These results offer clues for deriving marker candidates for accurate
neuroblastoma risk diagnosis.
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bioinformatics analysis16 glycansmarker candidatessugar abundanceprotein residuesglycosylationNLFprotein modificationneuroblastoma risk diagnosissialylated glycan structuresNeuroblastoma Cell LinesAlthough MYCN amplificationresults offer cluesglycomic markersmonosaccharide countSY 5Y samplesnonsialylated glycansproteomic studiesprecancerous conditions
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